Microinjection Dispenser Controller (Part 2, Construction done)

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Continued from: part 1 This is more of a quick update, this weekend I finished the mechanical and electrical construction of my microinjection dispenser controller. Back Panel So I finished the back panel as mentioned in part 1. The BNC at the top gives a TTL marker signal and I also added a 4mm banana socket for grounding. Remote In order to trigger it I wanted the possibility of a small remote with a button instead of having to press the button on the front panel every time.

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Microinjection Dispenser Controller (Part 1)

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Continued in: part 2 I saw some people building open-source microinjection dispensers like OpenSpritzer and PuffAdder a while back and thought “hey I can easily build this”. And so as an excercise I did. This is a pressure ejection microconjection dispension controller. It works by having a regulated pneumatic pressure being dosed by opening a fast acting solenoid valve for a specified amount of time. The relation of the pressure and the open time of the solenoid then dictate how much volume flow will be ejected.

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DIN912 Stainless Steel M4 Screw Weights

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DIN912 M4 screw weights, sample size \(n=10\), weighted on my Sauter L10 which has a declared error of \(e=0.1\rm{g}\) and \(d=0.1\rm{g}\). Conducted at room temperature \(T=21^{\circ}\). I did this on my Sauter scale because it is a direct reading scale making it faster to handle, than my other (more precise) laboratory scales, especially considering the sample size of 10 I’m using here.

Values in between indicators were rounded up to the next higher 10s of milligrams.

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Making a LOMO Power Supply Safe(er)

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As mentioned in the MBS-10 (МБС-10) post the standard LOMO power supply is not safe by modern standards. It uses a non-earthed metallic case and does not have fuses. I recently got this supply together with my new LOMO R15 (ЛОМО Р15) where the prior owner cut off the standard europlug and added a Schutzkontaktstecker using screw terminals. It is as safe as it looks: While disassembling it I also noticed that the shrinking tube was improperly sized, exposing live 230V terminals:

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LOMO R15 (ЛОМО Р15)

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I recently got this LOMO R15 (ЛОМО Р15) as I wasn’t satisfied with the accessories my Hertel & Reuss microscopes have and the virtual non-availability of further accessories for those on eBay. So I decided to go with a microscope I can get more easily upgrades for. As visible from my earlier posts regarding this: LOMO ST-12 (ЛОМО СТ-12) Repair Cleaning a LOMO R15 (ЛОМО Р15) Frame LOMO OI-35 (ЛОМО ОИ-35) Repair There was some cleaning and mechanical maintenance required, to get it back into operation.

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LOMO OI-35 (ЛОМО ОИ-35) Repair

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I got this together with my new LOMO R15 (ЛОМО Р15), the iris diaphragm inside was broken, so here a quick note on how I repaired it. I started with dissembling everything, in order to get the diaphragm out the two levers need to be unscrewed as well as a small set screw on the bottom needs to be removed. I also disassembled the mirror part for cleaning. The diaphragm was completely sticky due to dried up grease, so I carefully disassembled it and removed all grease remains using WD-40.

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Analog Threshold Relay

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This is part of my readout electronics system. This is one of the oldest modules I built that I haven’t desoldered so far. It is an analog threshold relay with voltage reference and amplification. There is a 10V reference voltage feeding into the 10-turn potentiometer on the front panel, this selects the threshold voltage. A lever switch selects whether the input signal is amplified by x10 or just passed through to the comparator.

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Cleaning a LOMO R15 (ЛОМО Р15) Frame

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So as mentioned in the preceding article about my LOMO ST-12 (ЛОМО СТ-12) Repair I got a new LOMO microscope! A LOMO R15 (ЛОМО Р15) to be precise, in this post I briefly go over cleaning the main frame of it, as mine was dirty and the mechanical parts were hard to operate. The green stuff is a grease spillage, where the grease liquefied by heating up and spilled all over the microscope.

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LOMO ST-12 (ЛОМО СТ-12) Repair

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So I recently got a LOMO R-15 (ЛОМО Р-15) microscope, that badly needs some cleaning and servicing. So let’s start with the XY stage which is a LOMO ST-12. As can be seen from this image it is slightly rusty and really dirty. I basically started by disassembling it and cleaning all parts in the process. There was quite a lot of excess grease and oil on it implying that someone had a go with this before.

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Universal Temperature Controller

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I got one of these cheap “REX C100” temperature controllers from Amazon a while back. So I figured I’ll make it usable by building a desk temperature controller with it. Modifying the Controller By default when you buy cheap temperature controllers like this it’s essentially Russian roulette on whether you get one with a SSR driver or a relay output. I happened to get one with a SSR driver, but need a relay for this.

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